解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::RNA copies, synthesized in vitro, of the virulent satellite (RNA C) of turnip crinkle virus (TCV) infect plants when coinoculated with helper virus RNA. RNA C is a small linear RNA of about 355 bases which intensifies TCV symptoms in infected plants. Full-length cDNA copies of the satellite were inserted in an express...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90445-4
更新日期:1987-01-01 00:00:00
abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90437-5
更新日期:1987-01-01 00:00:00
abstract::Monoclonal antibodies have been produced and used to map the functional topography of the surface proteins of rhesus rotavirus (RRV) that mediate viral neutralization. Ten monoclonal antibodies directed to VP7 were studied in neutralization assays and competitive binding studies. A large neutralization domain with sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90205-9
更新日期:1986-12-01 00:00:00
abstract::Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90207-2
更新日期:1986-12-01 00:00:00
abstract::HAV particles isolated from infected cells banded at buoyant densities of 1.42, 1.32, and 1.20 g/ml, and distinctive protein patterns were established by gel electrophoresis and reverse phase high performance liquid chromatography. The relatively higher amounts of p30 in particles with lower buoyant densities suggest ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90234-5
更新日期:1986-12-01 00:00:00
abstract::Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90216-3
更新日期:1986-12-01 00:00:00
abstract::Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90232-1
更新日期:1986-12-01 00:00:00
abstract::Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90179-0
更新日期:1986-11-01 00:00:00
abstract::An open reading frame which encodes at least 90% of the adenovirus type 2 DNA polymerase gene was cloned behind the SP6 promoter and transcribed in vitro using the SP6 RNA polymerase. The resultant RNA was translated in a rabbit reticulocyte cell free system. In addition to the translation of a 120-kDa protein corresp...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90181-9
更新日期:1986-11-01 00:00:00
abstract::An immunological relationship was detected between the coat protein of the common (U1) strain of tobacco mosaic virus (TMV) and the large subunit of the ubiquitous CO2-fixing host enzyme, ribulose-1,5-biphosphate carboxylase (RuBisCo). When assayed by Western immunoblotting or indirect ELISA, polyclonal antisera to TM...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90186-8
更新日期:1986-11-01 00:00:00
abstract::Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing spec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90465-4
更新日期:1986-10-30 00:00:00
abstract::The phosphorylation and transcriptional competence of the free cytoplasmic form and the virion form of NS protein of vesicular stomatitis virus (VSV-Indiana/Mudd-Summers) were compared. NS protein is known to exist in two distinct phosphorylated states, NS1 and NS2, that are resolvable by gel electrophoresis. In vitro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90452-6
更新日期:1986-10-30 00:00:00
abstract::Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90457-5
更新日期:1986-10-30 00:00:00
abstract::Extra MMTV proviruses in T-cell leukemias of GR and C57/BL10 mice contain alterations in their long terminal repeat (LTR) sequence. The four different leukemias studied contain different deletions, but common hallmarks were observed around the recombination sites. At the 5' end of the deletions we observed a common no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90431-9
更新日期:1986-10-15 00:00:00
abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90439-3
更新日期:1986-10-15 00:00:00
abstract::Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90437-x
更新日期:1986-10-15 00:00:00
abstract::The budded phenotype of Autograha californica nuclear polyhedrosis virus (AcNPV BV) has a 64K glycoprotein in its envelope which is important in the ability of the virus to functionally enter host cells. Tunicamycin (TM), an inhibitor of N-linked glycosylation, was used to determine whether the sugar moiety was essent...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90443-5
更新日期:1986-10-15 00:00:00
abstract::We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90030-9
更新日期:1986-09-01 00:00:00
abstract::The nucleotide sequence was determined of a 1.6-kb restriction fragment containing the polyhedrin gene of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV). In addition, both the 5' and 3' termini of the polyhedrin mRNA were located in the flanking sequences using S1 mapping procedures. 5' Le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90031-0
更新日期:1986-09-01 00:00:00
abstract::The molecular cloning of the DNA provirus of Mason-Pfizer monkey virus (M-PMV) is described. Fourteen independent clones of integrated M-PMV proviruses were isolated from a human embryo kidney cell line that had been previously derived from a single cell clone infected with M-PMV. Characterization of these clones for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90023-1
更新日期:1986-09-01 00:00:00
abstract::Nucleotide sequencing studies (D. J. McGeoch, A. Dolan, S. Donald, and F. Rixon, 1985, J. Mol. Biol. 181, 1-14) have indicated that herpes simplex virus type 1 (HSV-1) has a coding sequence, referred to as US7, between the genes for the glycoproteins D and E (gD and gE). Northern blot analysis and nucleotide sequencin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90002-4
更新日期:1986-08-01 00:00:00
abstract::Primary varicella-zoster virus (VZV) infection of humans may result in latent infection of sensory neurons in the peripheral nervous system. To examine the interaction of VZV with the sensory neuron we infected immunochemically defined human neurons with cell-associated VZV. Utilizing double-label immunofluorescence t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90141-8
更新日期:1986-07-30 00:00:00
abstract::We determined the effect of the overproduction of viral nonstructural proteins on alphavirus plus-strand and minus-strand RNA synthesis. Because alphavirus minus-strand synthesis ceases normally at 3 to 4 hr postinfection and requires continuous protein synthesis [D. L. Sawicki and S. G. Sawicki, J. Virol. 34, 108-118...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90157-1
更新日期:1986-07-30 00:00:00
abstract::Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer via in vivo recombination. The mutants were identified using a replica filter plating technique by in situ ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90132-7
更新日期:1986-07-30 00:00:00
abstract::This report shows that (S)CARNA 5, the satellite of cucumber mosaic virus (CMV) strain S, directs the synthesis of two small proteins in a wheat germ cell-free translation system. The two proteins are produced by the translation of both (S)CARNA 5 purified from CMV virions and the (+) strand purified from double-stran...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90147-9
更新日期:1986-07-30 00:00:00
abstract::Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90138-8
更新日期:1986-07-30 00:00:00
abstract::A cDNA clone containing all of the 26 S mRNA coding region of the RNA genome of Venezuelan equine encephalitis (VEE) virus, virulent strain Trinidad donkey (TRD), has been constructed and sequenced. The nucleotide and deduced amino acid sequences of the 26 S RNA of VEE virus conform to the general organization of the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90142-x
更新日期:1986-07-30 00:00:00
abstract::In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90394-6
更新日期:1986-07-15 00:00:00
abstract::The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90380-6
更新日期:1986-07-15 00:00:00
abstract::In situ hybridization of viral DNA fragments to metaphase chromosomes of two chicken T-lymphoblastoid cell lines (MDCC) demonstrated that Marek's disease virus DNA was located in the short arm of chromosome 2 of MDCC-MSB1 and MDCC-RP1 cells and in the short arm of chromosome 4 of MDCC-MSB1 cells. The MDV DNA sequences...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90390-9
更新日期:1986-07-15 00:00:00
abstract::Bacteriophage T4 tails contain a set of extended protein molecules in the central channel of the tail tube through which the DNA must exit during infection. Treatment of tails with guanidine hydrochloride separates the baseplates, leaving the tail tube and several specific tube-associated proteins. Methods were develo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90051-6
更新日期:1986-06-01 00:00:00
abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90060-7
更新日期:1986-06-01 00:00:00
abstract::Viruses were recovered from HeLa S3 cells and African green monkey kidney (AGMK) cells transfected with an infectious cDNA clone of poliovirus vaccine Sabin 1 strain. The viruses recovered from the different DNA-transfected cells were tested for the biological characteristics of temperature sensitivity (rct marker), p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90100-5
更新日期:1986-05-01 00:00:00
abstract::The complete nucleotide sequence of cloned cDNAs corresponding to the full length of the mRNA encoding the measles virus hemagglutinin (H) protein has been determined. the mRNA contains a single large open reading frame which is capable of encoding a protein of 617 amino acids with a molecular mass of 69,250 Da. The d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90312-0
更新日期:1986-04-30 00:00:00
abstract::RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90305-3
更新日期:1986-04-30 00:00:00
abstract::Analysis of expression of the Friend murine leukemia virus (F-MuLV) and of the spleen focus forming virus (SFFV) has been undertaken in highly malignant interferon (IFN)-sensitive (745) and IFN-resistant (3Cl-8) Friend leukemia cells (FLC), serially passaged intraperitoneally in DBA/2 mice. In vivo passaged 745 cells,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90304-1
更新日期:1986-04-30 00:00:00
abstract::Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90266-7
更新日期:1986-04-15 00:00:00
abstract::The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90287-4
更新日期:1986-04-15 00:00:00
abstract::Murine monoclonal antibodies were developed against the protein products produced by murine C127 cells which had been transfected with a recombinant plasmid clone containing the human T-cell leukemia (lymphotropic) virus type I (HTLV-I) proviral DNA coding regions for part of env, px, and the 3' LTR. Four antibodies w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90270-9
更新日期:1986-04-15 00:00:00
abstract::The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90128-5
更新日期:1986-03-01 00:00:00